Parasubicular axons target layer 2 pyramidal cell patches in medial entorhinal cortex. A, Left, Tangential section stained for calbindin (green) revealing patches of calbindin-positive pyramidal neurons. Middle, Same section as left processed to reveal the tracer BDA (red). *Location of the parasubicular injection site. Right, Overlay. Scale bar, 150 μm. B, Same as A but at higher magnification. Scale bar, 150 μm. C, High-magnification view of a single patch. D, Dorsal; L, lateral; M, medial; V, ventral.