Effect of the BK channel opener BMS-204352 on synaptic transmission and synaptic depression. A, BMS-204352 (10 μm) had no effect on amplitude of evoked EPSCs (p = 0.937). B, Sample traces of evoked EPSCs by a standard paired pulse stimulation protocol. C, BMS-204352 had also no effect on the PPR (p = 0.413). D, Sample traces showing EPSC1 and EPSC50 evoked by burst stimulation under different drug conditions. E, Bath application of BMS-204352 seemed to attenuate synaptic depression when EPSC amplitudes were normalized to the first amplitude of each condition (p = 0.001). F, Because the first EPSC amplitude during BMS-204352 application tended to be 20% lower than under control conditions, BMS-204352 amplitudes were normalized to EPSC1 of the control condition for each cell. This revealed that BMS-204352 did not change synaptic depression (p = 0.291). Data are mean ± SEM.