TM-mediated tumor-cell interconnections and radioresistance. a, Ratio of TM-connected/non-TM-connected S24 shControl and shTtyh1 GBMSCs 20 and 40 d after implantation (n = 3 mice per group; Mann–Whitney tests). b, Left, Representative 3D MPLSM images of S24 shControl and shTtyh1 tumors before (Pre Rad) and 7 d after (Post Rad) irradiation (z = 51 μm). Right, Corresponding quantification shows absolute cell numbers in a volume of 276 * 10³ μm³ before and 7 d after irradiation (n = 3 animals per group; ANOVA on ranks). c, Ratio of cell number 7 d after/before irradiation with respect to TM connectivity (n = 3 mice per group; ANOVA). a–c, Data obtained by in vivo MPLSM. *p < 0.05; **p < 0.01; ***p < 0.001.