Effects of SNI on TNF-α and BDNF expression in hippocampus and spinal dorsal horn. A, Western blots showing the levels of TNF-α (26 kDa) and BDNF (18 kDa) in contralateral (C) and ipsilateral (I) hippocampus of WT mice in sham and SNI groups: n = 5, TNF-α, p < 0.001 (I), p = 0.009 (C); BDNF, p < 0.001 (I), p = 0.021 (C). B, Levels of TNF-α and BDNF in contralateral and ipsilateral spinal dorsal horn in the indicated groups: n = 5, TNF-α, p = 0.014 (I), p = 0.662 (C); BDNF, p = 0.032 (I), p = 0.737 (C). RelOD, Relative optical density. Values are the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus sham groups. Data for all bar graphs were analyzed using Student's t test.