NS9283 potentiates the currents induced by the exogenous application of nicotinic agonists. Agonists were applied until the response reached steady state before adding NS9283 (10 μm) for ∼10 min. A, Carbachol 5 μm. B, Nicotine 0.2 μm. C, Cytisine 10 nm. D, saz-A 2 nm. E, Summary of the effects obtained in multiple experiments with various concentrations of agonists: carbachol (2, 5, and 10 μm), nicotine (0.2 and 1 μm), cytisine (10 nm), and saz-A (2 nm). F, Mean values of the potentiation induced by NS9283 on the responses to the four agonists. Carbachol 2 μm: 2.1 ± 0.3 (n = 4). Nicotine 0.2 μm: 2.0 ± 0.1 (n = 3). Cytisine 10 nm: 3.3 ± 1.0 (n = 4). saz-A 2 nm: 4.0 ± 4.0 (n = 9).