Neostigmine prolongs the slow component of the eEPSC but does not increase its amplitude. A–C, Neostigmine was applied at 30 nm and then at 50 nm. A, B, Average of 10 EPSCs. A, 1p-EPSCs. B, 5p-EPSCs. C, Time course of the changes of τ₂^1p (C₁, open circles), τ₂^5p (C₁, filled circles), a₂^1p (C₂, open diamonds), and a₂^5p (C₂, filled diamonds) after addition of neostigmine. D, Bar graphs represent the average changes of τ₂ (D₁) and a₂ (D₂) induced by neostigmine (50 nm) for 1p-EPSCs and 5p-EPSCs. Neostigmine only increased τ₂, whereas NS9283 increased both τ₂ and a₂ (Fig. 1). **p < 0.01. ***p < 0.001.