Overexpression of dominant-negative SNX1 (SNX1-CC) results in the faster recycling of mGluR1. A, Representative images show that overexpression of SNX1-CC domain had no effect on the surface expression of myc-mGluR1. B, Quantitation of the effect of SNX1-CC overexpression on the surface expression of myc-mGluR1. C, Overexpression of SNX1-CC domain affected the ligand-mediated trafficking of myc-mGluR1 as shown in representative images. D, Quantitation also shows that SNX1-CC overexpression led to the altered trafficking of myc-mGluR1. E, Representative images showing the kinetics of ligand-induced trafficking of myc-mGluR1 in control and SNX1-CC overexpressed cells. F, G, In control cells, upon application of 100 μm R,S-DHPG internalization of myc-mGluR1 progressively increased till 30 min as shown by the quantitation of the endocytosis (F) as well as measurement of the surface myc-mGluR1 (G). H, I, On the other hand, in cells overexpressing SNX1-CC domain, the receptor showed maximum endocytosis at 5 min after 100 μm R,S-DHPG application and recycled back to the cell surface at 30 min, as shown by the quantitation of the endocytosis (H) and surface localized myc-mGluR1 (I). Results are presented as mean ± SEM collected from three independent experiments. Scale bar, 10 μm. ***p < 0.001, **p < 0.01, *p < 0.05; n.s., p > 0.05.