Knockdown of endogenous SNX1 results in the faster recycling of mGluR1. A, B, Western blot analyses (A) and quantitation of the Western blots (B), confirming that the endogenous SNX1 was efficiently knocked down by si-SNX1 in primary neurons. C, Representative images show that acute knockdown of endogenous SNX1 had no effect on the surface expression of myc-mGluR1. D, Quantitation of the effect of SNX1 knockdown on the surface expression of myc-mGluR1. E, Representative images showing that knockdown of endogenous SNX1 resulted in the recycling of myc-mGluR1 within 30 min after 100 μm R,S-DHPG application, whereas in control cells and si-control treated cells the receptor showed progressive increase in the internalization till 30 min post-ligand application. F1, F2, Quantitation of the endocytosis (F1) and surface myc-mGluR1 (F2) also suggested that in control cells the receptor showed maximum endocytosis at 30 min post-ligand application. G1, G2, Importantly, acute knockdown of endogenous SNX1 resulted in the recycling of the receptor to the cell surface within 30 min after 100 μm R,S-DHPG application as shown by the quantitation of the endocytosis (G1) and measurement of surface localized myc-mGluR1 (G2). H1, H2, Measurement of the endocytosis (H1) and surface myc-mGluR1 (H2) suggested that 100 μm R,S-DHPG induced trafficking of myc-mGluR1 in si-control treated cells was similar to the control cells. Results are presented as mean ± SEM collected from three independent experiments. Scale bar, 10 μm. ***p < 0.001, ** p < 0.01, *p < 0.05; n.s., p > 0.05.