SIMBA imaging of clathrin-coated pits in a live HeLa cell. (A) Overlay of 100 total internal reflection fluorescence image frames representing diffraction-limit image of mEos3.2- clathrin light chain (CLC). (B) Reconstructed super-resolution radial fluctuations image of mEos3.2-CLC. (C) Reconstructed SIMBA image of mEos3.2-CLC. Scale bars: 1 μm. (D), (E), and (F): Zoomed-in images of clathrin-coated pits (CCPs) marked by yellow boxes in (A), (B), and, (C) respectively. Scale bars: 200 nm.