Enriched expression of Neogenin in excitatory neurons in BLA. A, Enriched β-gal activity in BLA, SGZ, and VPM/VPL, but not in CeA. Top left, Diagram of coronal section. Top right, X-gal staining of brain slice of Neogenin-Lacz heterozygous mice. Bottom, Enlarged image of amygdala (amyg). Scale bars: Top, 1 mm; Bottom, 200 μm. B, Colocalization of β-gal with excitatory neuron marker Camkii. Sections were stained with antibodies against β-gal and Camkii. Bottom, Enlarged images of areas in dotted square. Arrow indicates f1, Camkii⁺β-gal⁺. Arrowhead indicates f2, Camkii⁺ only. Empty arrowhead indicates f3, Camkii⁺ cells with β-gal dots. Scale bars: Top, 120 μm; Bottom, 20 μm. C, Fewer interneurons were labeled by β-gal. Brain sections of Neogenin-Lacz;GAD67-GFP mice were stained with antibodies against β-gal and NeuN. Bottom, Enlarged images of areas in dotted square. Arrow indicates f1, GFP⁺β-gal⁺. Arrowhead indicates f2, GFP⁺ only. Scale bars: Top, 120 μm; Bottom, 20 μm. D, Quantitative analysis of data in B, C. Only cells whose entire soma were labeled by Camkii or GFP were scored. n = 8 slices from 3 mice.