Morphological characterization of GFAP-Neo^−/− amygdala. A, Breeding diagram: Neogenin^f/f (Neo^f/f) mice were crossed with GFAP-Cre mice to generate GFAP-Cre;Neogenin^f/f (GFAP-Neo^−/−) mice. B, Neogenin expression was dramatically decreased in amygdala of GFAP-Neo^−/− mice. β-actin serves as loading control. Left, Representative blots. Right, Quantitative data. n = 3 pairs of Neo^f/f and GFAP-Neo^−/− mice. Paired Student's t test: **p < 0.01. C, Similar axon projections in Neogenin mutant brain. acp, Posterior part of anterior commissure; ec, external capsule Scale bar, 1 mm. D, Rostral sections of Neo^f/f (top) and GFAP-Neo^−/− (bottom) amygdala were stained for neuron marker NeuN and interneuron marker PV. Scale bar, 100 μm. E, Caudal sections of Neo^f/f (top) and GFAP-Neo^−/− (bottom) amygdala were stained for neuron marker NeuN and interneuron marker PV. Scale bar, 100 μm. F, Quantification of NeuN⁺ cells in the BLA regions. n = 13 slices from 3 Neo^f/f mice; n = 17 slices from 3 GFAP-Neo^−/− mice. Student's t test: p > 0.05. G, Quantification of PV⁺ cells in the BLA regions. n = 13 slices from 3 Neo^f/f mice; n = 17 slices from 3 GFAP-Neo^−/− mice. Student's t test: p > 0.05.