Unaffected neuronal intrinsic property by Cre virus infection and Neogenin mutation. A, A schematic of virus injection. CMV promoter-driven Cre virus was injected bilaterally into BLA region. B, Expression of GFP in virus-injected mice. Amygdala sections were stained with GFP and NeuN antibodies 3 weeks after stereotaxic microinjection of AAV-Cre-GFP virus. Scale bar, 100 μm. C, Reduced Neogenin expression in amygdala of Neo^f/f;td⁺ mice injected with AAV-Cre-GFP virus, compared with that from td⁺ mice. β-actin serves as loading control. Shown are representative blots. 1, 3, 5: samples from td⁺ mice; 2, 4, 6: samples from Neo^f/f;td⁺ mice. Hypo, Hypothalamus; Pir, piriform cortex; Amyg, amygdala. D, Quantitative analysis of data in C. n = 3 pairs of td⁺ and Neo^f/f;td⁺ mice for Hypo and Pir groups; n = 4 pairs for Amyg group. Paired Student's t test: Hypo and Pir groups, p > 0.05; Amyg group, *p < 0.05. E, Diagram of the paired-recording configuration. Dark orange represents a Cre⁺ pyramidal neuron. Cortical input pathway was stimulated. F, Representative traces in BLA pyramidal neurons in response to current injection of −50 pA and 50 pA, respectively. Scale bars, 40 ms, 50 pA. G, Characterization of resting membrane potential (RMP) of pyramidal neurons. n = 6 neuron pairs from 3 td⁺ mice; n = 7 neuron pairs from 3 Neo^f/f;td⁺ mice. Paired t test: p > 0.05. H, Characterization of membrane input resistance (Rin) of pyramidal neurons. n = 6 neuron pairs from 3 td⁺ mice; n = 7 neuron pairs from 3 Neo^f/f;td⁺ mice. Student's t test: p > 0.05. I, Firing frequency was not altered by injecting 50 pA current. n = 6 neuron pairs from 3 td⁺ mice; n = 7 neuron pairs from 3 Neo^f/f;td⁺ mice. Student's t test: p > 0.05.