Neogenin in BLA neurons regulates excitatory synaptic transmission in a cell-autonomous manner. A, Representative AMPARs (down) and NMDARs (up)-mediated EPSC traces recorded from Cre⁻/Cre⁺ neuronal pairs in the BLA in td⁺ and Neo^f/f;td⁺ mice injected with AAV-Cre-GFP virus. Scale bars, 40 ms, 50 pA. B, Quantification of AMPAR-mediated EPSC amplitude, which was normalized to the mean EPSC amplitude of Cre⁻ neurons. n = 11 pairs from 4 td⁺ mice; n = 16 pairs from 5 Neo^f/f;td⁺ mice. Paired Student's t test: *p < 0.05. C, Quantification of NMDAR-mediated EPSC amplitude, which was normalized to the mean EPSC amplitude of Cre⁻ neurons. n = 11 pairs from 4 td⁺ mice; n = 15 pairs from 5 Neo^f/f;td⁺ mice. Paired Student's t test: *p < 0.05. D, Similar AMPA/NMDA ratios between Cre⁻ and Cre⁺ neurons in td⁺ and Neo^f/f;td⁺ mice. n = 9 pairs from 4 td⁺ mice; n = 10 pairs from 4 Neo^f/f;td⁺ mice. Paired Student's t test: p > 0.05. E, Representative mEPSC traces. Scale bars, 1 s, 10 pA. F, Reduced mEPSC frequency in mutant mice. n = 9 pairs from 4 td⁺ mice; n = 10 pairs from 4 Neo^f/f;td⁺ mice. Paired Student's t test: for td⁺ group, p > 0.05; for Neo^f/f;td⁺ group, *p < 0.05. G, Similar mEPSC amplitudes. n = 9 pairs from 4 td⁺ mice; n = 10 pairs from 4 Neo^f/f;td⁺ mice. Paired Student's t test: p > 0.05. H, Diagram of tone-cued fear conditioning, as described above. I, Reduced freezing time in the presence of tone in Neo^f/f;td⁺ mice with Cre virus. n = 12 mice for each group. Student's t test: *p < 0.05.