Tmods in dendritic spines of hippocampal neurons in culture. A, Immunostaining of endogenous Tmod1 and Tmod2 (green), together with phalloidin-labeled dendritic spines and PSD-95 (magenta). Scale bar, 5 μm. B, Representative immunofluorescence images of Tmod1 and Tmod2 in select dendritic regions of extracted and nonextracted hippocampal neurons. Scale bar, 2.5 μm. C, Bar graph depicts the mean fluorescence intensity of Tmod1 and Tmod2 in the dendritic shaft of nonextracted (control) and extracted neurons. The fluorescence intensity values of Tmod1 and Tmod2 in the shaft are normalized to the mean corresponding value in the spine. An unpaired Student's t test was used to compare the nonextracted vs extracted mean intensity values for Tmod1 and Tmod2. *Statistical difference from the control. For shaft fluorescence: t(df) = 2.44(6), p^Tmod1-ext. = 0.08, t(df) = 2.575(5), p^Tmod2-ext. = 0.06. Error bars represent the SEM. D, Bar graphs depict the mean fluorescence intensity of Tmod1 and Tmod2 in dendritic spines of nonextracted (control) and extracted neurons. Extracted values are normalized to the nonextracted values of Tmod1 and Tmod2, respectively. N_spines = 196 and 233, for Tmod1 and Tmod2, respectively. N_shaft = 180 and 173, for Tmod1 and Tmod2, respectively.