Subspine localization of Tmod1 and Tmod2. A, 3D-SIM images of Tmod1/Tmod2 and F-actin in dendritic spines demonstrate the subspine localization of Tmods to the neck and base of the spine head. B, Bar graphs represent mean raw integrated density Tmod/F-actin ratios in the proximal and distal region of the spine head as well as the spine neck. C, STED image showing the distribution of Tmod1 or Tmod2 (green) and F-actin (magenta) in spines. D, STED image showing the distributions of Tmod1 (green) and Tmod2 (magenta) in the same dendritic protrusion. Scale bars: top, 0.5 μm; bottom, 1 μm.