Table 1.
Effect of neuronal NR4A1 on mitochondria-regulatory gene expression
| Treatments | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DRP1 | MFN1 | MFN2 | FIS1 | OPA1 | UCP2 | UCP4 | UCP5 | |||||||||
| % | SE | % | SE | % | SE | % | SE | % | SE | % | SE | % | SE | % | SE | |
| Ratio (NR4A1/GFP) | ||||||||||||||||
| Untreated controls | 127.6 | 15.3 | 128.7 | 28.4 | 113.5 | 13.4 | 106.6 | 6.8 | 111.0 | 8.4 | 22.3** | 14.9 | 157.5** | 26.3 | 112.8 | 17.8 |
| 25 ng/ml BDNF | 119.5 | 16.9 | 132.3 | 22.0 | 104.8 | 11.9 | 97.9 | 8.8 | 75.7 | 8.3 | 87.4 | 10.6 | 126.4 | 16.2 | 77.8 | 11.8 |
| 25 mm glutamate | 95.9 | 7.9 | 127.9 | 10.2 | 96.7 | 20.1 | 129.6 | 8.2 | 107.8 | 11.0 | 85.6 | 17.4 | 157.9 | 30.5 | 88.9 | 14.5 |
| 100 mm glutamate | 121.3 | 16.6 | 70.1* | 3.5 | 90.1 | 4.5 | 159.8* | 20.7 | 155.5* | 24.7 | 61.5 | 28.6 | 176.9* | 14.9 | 98.2 | 9.2 |
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Messenger RNA levels determined by qPCR on total RNA extracts of primary cortical neurons (DIV14 with 10 d of doxycycline treatment). Data are expressed as a percentage of GFP controls (mean ± SE of N = 4 independent experiments). Unpaired t test for comparing the effect of NR4A1 with GFP in untreated control cells.
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↵**p < 0.036, for comparing effect of NR4A1 with GFP in BDNF-treated cells (25 ng/ml for 3 h).
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↵*p < 0.045 for comparing the effect of NR4A1 with GFP in glutamate-treated cells (100 μm for 3 h).
