Genetic inactivation of Dg does not result in body posture abnormality. While tw mutants show conspicuous body torsion resulting in displacement of dorsal tracheal trunks (arrows) and a rotated position of the last segment (bracket) with posterior spiracles (asterisks), Dg mutants show no torsion and are indistinguishable from wild-type control (WT). Representative images of fixed first instar larvae obtained with transmitted light illumination. Dg⁻, Dg⁰⁸⁶/Dg⁰⁸⁶; tw⁻, tw¹/tw¹(or tw¹/Y); WT, wild-type control with matching genetic background (Canton-S). More than 20 larvae were analyzed for each genotype.