Dural administration of CGRP produces facial hypersensitivity and priming in female, but not in male, rats. Baseline facial withdrawal thresholds were determined before dural stimulation with 100 μm CGRP (A) (n = 10 females, n = 7 males), 0.1 μm CGRP (n = 4 females) (B), or vehicle (n = 5 females, n = 7 males). A dural injection of pH 7.0 SIF was given in all groups at day 5 following either dural CGRP or dural vehicle. Two-way ANOVA followed by Bonferroni post hoc test indicated significant differences in females treated with 100 μm CGRP (F_(27,250) = 2.591, p < 0.0001) and in females treated with 0.1 μm CGRP (F_(27,250) = 2.591, ****p < 0.0001). These data are represented as means ± SEM. *p < .05, **p < .01, ***p < .001.