PTX-induced TNF and CXCL1 expression and release are impaired in male Tlr9 mutant mice. A–H, ELISA testing results of concentrations of TNF and CXCL1 in peritoneal macrophage culture medium (A, B; n = 3 culture per group, 1 × 10⁵ cells per culture), serum (C, D; n = 5 per group, 100 μl serum per sample), peritoneal fluid (E, F; n = 5, 6 ml per mice), and DRG tissue lysate (G, H; n = 5 per group, 3 DRG tissues collected per mice). Compared with the control or naive group with the same genetic background (two-way ANOVA with Bonferroni's post hoc test): ***p < 0.001; **p < 0.01; *p < 0.05. Compared with WT mice group (two-way ANOVA with Bonferroni's post hoc test): ^###p < 0.001; ^##p < 0.01; ^#p < 0.05. Data are mean ± SEM.