Temperature effects on hair cell passive membrane properties. A1, Inward currents were triggered by a small hyperpolarization from −90 to −100 mV at 23°C (black) and 31°C (red). The size of the current was the difference between baseline and the plateau inward current (dashed lines). B1, Hyperpolarized membrane potential in response to a −30 pA current injection under current clamp was recorded at 23°C (black) and 31°C (red). The change in membrane potential was calculated by subtracting baseline from plateau (dashed lines). A2, Using voltage-clamp measurements and R_in = ΔV/ΔI, the membrane input resistance (R_in) was lower at high temperature (p = 0.0006, n = 7). B2, Current-clamp measurements also showed that R_in decreased at high temperature (p = 0.0031, n = 7). B3, Membrane time constant (τ) was estimated by fitting a single exponential to a 40 ms time window of the voltage response following the initial current injection (B1, superimposed blue dashed lines). τ was faster at high temperature (p < 0.0001, n = 7). B4, C_m calculated using C_m = τ/R_in did not show significant change at high temperature (p = 0.2506, n = 7). A2, B2–B4, Dots represent individual values; open circles represent mean; bars represent SEM. **p < 0.01, ***p < 0.001, ****p < 0.0001.