Abnormal MT organization in Neo1a- and Rgma-depleted embryos. A–C, Immunofluorescent detection of MTs in transverse hindbrain sections of 4–5 som control (A), neo1a MO2-injected (B), and rgma MO2-injected (C) embryos labeled using anti-β-tubulin antibodies. Nuclei were counterstained with DAPI (red). Double arrow indicates axis of NEC elongation and radial intercalation. Scale bar, 50 μm. A′–C′, Insets, Higher magnification of boxed areas in A–C. Arrows indicate elongated MTs. Arrowheads indicate bundled and short MTs. Scale bar, 25 μm.