Examples and schematic of experimental DGC recordings. A, Timeline of experimental protocol. EB cells were labeled at P7; AB cells were labeled at P60. All rats received SE or sham treatment at P56 and recordings were made between three and six months later (at P150–P240) to allow development of spontaneous recurrent seizures in pilocarpine-treated rats. An IR-DIC image of a patch-clamped DGC with GFP epifluorescence overlay and an example of biocytin staining are shown. B, Schematic diagram of the location for all DGCs that were recorded in each experimental category. GCL, granule cell layer. C, Representative images of biocytin-filled DGCs for each morphological category (i: normotopic morphology; ii, iii: ectopic location; iv: HBD+ morphology). Note that all images are from SE tissue. D, Confocal images of immunofluorescence double labeling of the dentate gyrus two months after pilocarpine treatment. In the left panel, GFP-labeled (green) hilar ectopic DGCs co-express Prox1 (red; the yellow arrows in inset denote double-labeled cells). In the right panel, GFP-labeled hilar ectopic DGCs do not co-express GABA (red). Cell nuclei are labeled with Hoechst (blue). Scale bar in D = 30 μm (C) 100 μm (D) 50 μm (D, insets).