GABA_Aα6 subunit expression is lower in Pclo^gt/gt than in Pclo^wt/wt. A, Representative images of sagittal sections of Pclo^wt/wt and Pclo^gt/gt cerebella at 3 months of age stained with antibodies against the GABA_A subunit α6, VGluT2, and MAP2. There are decreased levels of GABA_Aα6 in the GCL of Pclo^gt/gt animals compared with Pclo^wt/wt controls, quantified in B. B, Quantification of GABA_A subunit α6, measured by the average intensity of antibody staining in images taken from the GCL of the cerebellum, normalized to MAP2 antibody intensities for the same image (Pclo^wt/wt = 0.661 ± 0.0479 a.u., n = 45 images from 3 individual animals; Pclo^gt/gt = 0.443 ± 0.26 a.u., n = 44 images from 3 individual animals; Mann–Whitney U test, U = 589, ***p = 0.0009). Scale bars, 20 μm. Error bars indicate SEM. Data points represent images taken from 4 sections per animal. Animals used in this experiment are as follows: wt10, wt11, wt13; ko9-ko11.