A microfluidics device for presenting larval zebrafish with water flow. A, 3D-printed device, with one large channel and two small channels through which water flows. The flow of water through the channels (for an h-t flow stimulus) is indicated by arrows, and the position and orientation of the larva is indicated with a black arrow. B, Micro-PIV analysis of the water flow in the chamber, fish is for illustration only. The traces (bottom) indicate the speed of the flow along the width of the channel. C, Raster plot of the 65,000 fluorescent traces extracted from the 13 fish (left), the direction and timing of the flow is indicated by the colored bars at the bottom (colors match B). D, The raster plots for 6510 flow responsive neurons (after r2 filtering), grouped by similarity to one another.
