Excitatory hM3Dq DREADDs validation in ACC. Electrophysiological effect of bath application of CNO in slice. A, Representative photomicrograph showing hM3Dq-mCherry DREADDs under CaMKIIα in a recording site of a 400 µm slice in ACC. B, Spontaneous ACC activity in a Gq DREADD-expressing slice before (top) and after 20 min bath application of 10 mm CNO (bottom). CNO-induced spontaneous bursting was seen in 4 of 6 Gq DREADD-expressing slices from 3 rats. C, In the two Gq DREADD-expressing slices that failed to show spontaneous bursting, there was a decrease in the threshold for stimulation-induced postsynaptic responses. Plot represents results from one of these slices before (baseline) and after CNO application. D, Summary of responses to CNO in Gq DREADD-expressing and control slices. CNO did not elicit either spontaneous bursting or decrease threshold for evoked responses in all three of the control (nontransfected) slices from 3 rats.
