Changes in global mRNA expression in SORLA TG hippocampus. A, PCA analysis of transcriptomic profiles from WT (black) and SORLA TG (blue) hippocampus from 3-month-old mice. B, Volcano plot represents differentially expressed transcripts in SORLA TG versus WT hippocampus. A total of 449 significantly upregulated (red) and 930 downregulated (blue) DEGs were identified (Log₂ fold change < −0.3 or > 0.3, p < 0.05). C, Selected KEGG pathways and GO BP categories, enriched in SORLA TG versus WT RNAseq datasets, were identified by GO DAVID analysis. Pathways relevant to signal transduction (KEGG) or neurite outgrowth (GO BP) are shown (see also Extended Data Fig. 7-1). D, Potential enhancers (black) and inhibitors (gray) of neurite outgrowth were selected from the KEGG or GO BP categories in C for validation by qRT-PCR. RNAseq profiles (left) and qRT-PCR validation (right, mean ± SE) are shown. The qRT-PCR profiles show expected upregulation (enhancers) or downregulation (inhibitors) in the SORLA TG hippocampus, with the exception of Kif26b.