Characterization of intrinsic markers for parasympathetic neurons. A, A', Representative images showing both Tom⁺ and GFP⁺ neurons in the NG of E10.5 Chat^Cre;R26^tdTom;Chat^GFP embryos (n = 3). B, Transversal section of the NG from E12.5 Chat^Cre;R26^tdTom embryos (n = 3). Arrowheads: tdTom⁺ axons within the NG. Arrows: NG-bypassing tdTom⁺ axons. Asterisks: tdTom⁺/Phox2b⁺ NG sensory neurons. C, Transversal section of the SG from E12.5 embryos (n = 3) showing GFP⁺ and tdTom⁺ sympathetic neurons (TH⁺). D, D', Transversal section of E11.5 vGlut2^Cre;R26^tdTom embryos (n = 3) showing the expression of VAChT in motor and sympathetic (TH⁺) neurons, but not in DRG sensory neurons. E, Transversal section through the medulla of E12.5 embryos (n = 3) showing VAChT⁺ neurons within the DMN, but not in the NG. Scale bars: 500 µm (A), 100 µm (A', D, E), 25 µm (B, D'), 50 µm (C). NG, nodose ganglion; SG, sympathetic ganglion; SP, spinal cord; DRG, dorsal root ganglion; MN, motor neuron; DMN, dorsal motor nucleus of the vagus nerve.