Distinctive projection patterns of sensory and sympathetic axons within the E16.5 gut. A, Representative midgut segment from E16.5 vGlut2^Cre;R26^tdTom;Chat^GFP embryos (n = 3) showing sympathetic (TH⁺) and sensory (tdTom⁺) axons within the gut. A', Magnified view of the boxed area demonstrating distinctive projection patterns of sensory and sympathetic axons within the gut. Arrowheads indicate one enteric Tom⁺/GFP⁺ neuron. B, Transversal section of E16.5 midguts (n = 3) showing that both sensory (tdTom⁺) and sympathetic (TH⁺) axons project mainly along the myenteric plexus (GFP⁺), but not along the arteries (CD31⁺). Scale bars: 100 µm (A), 50 µm (A'), 100 µm (B).