Pharmacological blockade of enhanced memory retrieval by LC activation. A, Taste reactivity test in wild-type mice that received an intra-BLA infusion of PRAZ (0.1 or 0.5 μg/site) and PROP (0.4 or 2.0 μg/site). n = 7–10 for each mouse group. *p < 0.05 versus PBS infusion (Tukey's HSD test). B, Taste reactivity test showing the blockade of shortened response latency in the LC-activated Tg mice by the intra-BLA infusion of PRAZ and PROP at the lower doses (0.1 and 0.4 μg/site, respectively). n = 8 or 9 for each group in the Tg mice. n = 7 for each non-Tg group. *p < 0.05, **p < 0.01 versus PBS in the Tg mice (Tukey's HSD test). C, Locomotion test of the Tg mice that received the microinjection of PhAc (0.6%) into the LC and infusion of PRAZ and PROP (0.1 and 0.4 μg/site, respectively) into the BLA. After the habituation to the open field, the mice received the drug treatments, and then the total number of beam breaks (locomotor activity) during a 60 min period was monitored. n = 4 for each group in the Tg mice. Data are presented as the mean ± SEM. Individual data points are overlaid. D, Placement sites of the injection needles into the BLA (PBS, PRAZ of 0.1 and 0.5 μg/site, and PROP of 0.4 and 2.0 μg/site) for the taste reactivity test. E, F, Placement sites of the injection needles into the LC (0.6% PhAc) and BLA (PBS, 0.1 μg/site PRAZ, and 0.4 μg/site PROP) for the taste reactivity test (E) and locomotion test (F). The AP coordinates (in mm) are indicated. Scale bar, 1 mm.