Divergent input- and cell-specific control of excitatory NAc synapses by TRPV1R. A, Effects of the TRPV1R agonist capsaicin (10 μm) on oEPSCs at PFC, BLA, and vHipp synapses onto D1 (black circles) and D2 (white circles) MSNs. Capsaicin was applied for 30 min (black bar) after at least 10 min baseline recording. Capsaicin inhibited PFC-evoked oEPSCs similarly in both D1 (black) and D2 (white) MSNs. Capsaicin enhanced BLA-D1 MSN synapses but inhibited BLA-D2 MSN synapses. Finally, capsaicin inhibited vHipp-D1 MSN synapses and has no effect at vHipp-D2 MSN synapses. B, Individual and averaged oEPSCs amplitude experiments 10 min before (baseline) and 25–30 min after capsaicin; D1 MSNs, black circles: PFC n = 3, p = 0.0308, BLA n = 4, p = 0.0188, vHipp n = 5, p = 0.0010; D2 MSNs, white circles: PFC n = 3, p = 0.0135, BLA n = 3, p = 0.0087, vHipp n = 3, p = 0.0109 paired t test). C, Summary bar histogram of the maximal effects of capsaicin on oEPSCs at identified inputs onto D1 (PFC n = 3, BLA n = 4, vHipp n = 5, F_{(input 2,9)} = 157.4, p < 0.0001, one-way ANOVA) and D2 MSNs (PFC n = 3, BLA n = 3, vHipp n = 3, F_{(input 2, 6)} = 8.586, p = 0.0174, one-way ANOVA). D, Schematic view of the relative weight and effects of TRPV1R on oEPSCs at PFC, BLA and vHipp inputs onto D1 and D2 MSNs. n represents the number of mice. All data are shown as mean ± SEM. *p < 0.05.