TRPV1R delineates cell-type-specific LTD at amygdala synapses in the NAc core. A, Optical stimulation of BLA inputs (10 Hz for 10 min) induced a robust LTD in D2 (white circles, n = 4) but not in D1 MSNs (black circles, n = 5). B, Individual and averaged oEPSC amplitude before (baseline) and 25–30 min after LTD induction (LTD) in D1 and D2 MSNs (D1 MSNs, p = 0.3311, D2 MSNs, p = 0.0072, paired t test). C, Bath perfusion of the TRPV1R capsazepine (CPZ 10 μm) allowed the induction of LTD at BLA-D1 synapses (purple, n = 6; compared with control same as A). When CPZ was applied in the presence of the CB1R antagonist SR141716 (SR 5 μm) LTD induction was prevented (orange, n = 4). D, Individual and averaged oEPSCs amplitude before (baseline) and 25–30 min after LTD induction (LTD) in D1 MSNs in control, CPZ and CPZ + SR (D1 CPZ, p = 0.0056 paired t test; D1 CPZ + SR, p = 0.9737). E, Antagonism of TRPV1R converted LTD to LTP at BLA-D2 synapses (purple, n = 5). Bath application of the CB1R antagonist SR blocked LTD at BLA-D2 synapses (blue, n = 6). F, Individual and averaged oEPSCs amplitude before (baseline) and 25–30 min after LTD induction (LTD) in D2 MSNs with or without CPZ or SR (D2 CPZ, p = 0.0068; SR, p = 0.9546 paired t test). n represents the number of mice. All data are shown as mean ± SEM. *p < 0.05 paired t test.