Early-life inflammation selectively increased the probability of glutamate release in adult mice. A–D, Representative scaled traces of paired pulse recording of CA1 pyramidal cells evoked by the stimulation of afferent fibers in the Schaffer collateral (A, B) or temporoammonic (C, D) pathway (as indicated on the schematic on the left) from adult female (A, C) and male (B, D) mice exposed early in life to Veh (dark traces) or LPS (green/blue traces) at P14. Calibration: 100 pA, 50 ms. Scatter graphs on right show PPR of individual cells. Summary data display decreased PPR in CA1 pyramidal cells from both female (green symbols) and male (blue symbols) mice exposed to early inflammation compared with controls (white symbols) when the Schaffer collateral (A, B) was stimulated. However, when the temporoammonic pathway was stimulated (C, D), no significant differences in PPR were observed. Horizontal lines are the mean (n = 18–25 cells, 5–8 mice/group). SO, Stratum oriens; PTX, picrotoxin. **p < 0.01.