Changes of glutamatergic, Dbx1, and GABAergic neurons and GFAP cells in the VRC after bilateral injection of 6-OHDA or vehicle into the CPu. A, Photomicrographs showing a reduction of Vglut₂⁺ neurons in the medulla ventrolateral portion (bregma level: −7.2 mm). B, Rostro-caudal distribution of Vglut₂⁺ neurons in VRC. C, Photomicrographs showing a reduction of Dbx1⁺ neurons in the medulla ventrolateral portion. D, Rostro-caudal distribution of Dbx1⁺ neurons in VRC. E, Photomicrographs showing no change of Vgat⁺ neurons in the medulla ventrolateral portion. F, Rostro-caudal distribution of Vgat⁺ neurons in VRC; after bilateral injection of 6-OHDA (2) or vehicle (1) into the CPu (n = 4/group). G, Photomicrographs showing a reduction of Dbx1⁺/GFAP⁺ cells in the medulla ventrolateral portion. Rostro-caudal distribution of (H1) Dbx1⁺/GFAP^–, H2) Dbx1⁺/GFAP⁺ and (H3) Dbx1^-/GFAP⁺ cells after bilateral injection of 6-OHDA or vehicle into the CPu (n = 4/group). Two-way ANOVA, followed by Newman–Keuls test, p < 0.05. sc Amb, ambiguus nucleus semi-compact pars. Scale bars: 400 µm (A2–E2; applies to A1, A2, C1, C2, E1, E2), 100 µm (A2a; applies to A1a, A2a, C1a, C2a, E1a, E2a), and 50 µm (G2; applies to G1, G2).