Table 2.
List for primers
| 1. Primers for qPCR | ||
|---|---|---|
| Gene name | Species | Sequence 5′-3′ |
| Pen-2 | Mouse gDNA F | GAACTTGGAGCGGGTATCCA |
| Pen-2 | Mouse gDNA R | AGAGGTAGTCCCCAAGAGCG |
| Hes1 | Mouse gDNA F | CAACACGACACCGGACAAAC |
| Hes1 | Mouse gDNA R | CGGAGGTGCTTCACAGTCAT |
| GFAP | Mouse gDNA F | GCAAGAGACAGAGGAGTGGT |
| GFAP | Mouse gDNA R | CTCTTCCTGTTCGCGCATTT |
| Stat3 | Mouse gDNA F | CAGCTGGACACACGCTACCT |
| Stat3 | Mouse gDNA R | GGACTCTTGCAGGAATCGGCT |
| Nfia | Mouse gDNA F | TCTTGTGAAACGAAACGCCC |
| Nfia | Mouse gDNA R | GCCCACAGTGATTCTCAACC |
| Hdac3 | Mouse gDNA F | ATCGCCTGGCATTGACTCAT |
| Hdac3 | Mouse gDNA R | TGTAACGGGAGCAGAACTCG |
| 2. Primers for recombinant DNA | ||
| Gene name | amino acids | Sequence 5′-3′ |
| mNotch1 ICD-F | 1744-2531 | GTGCTGCTGTCCCGCAAGCGC |
| mNotch1 ICD-R | TTTAAATGCCTCTGGAATGTG | |
| mErbB4 ICD-F | 636-1292 | TACCCATGGACGGGCCATTC |
| mErbB4 ICD-R | TCACACCACAGTATTCCGGT | |
| mAPP ICD-F | 722-770 | ATGTTGAAGAAGAAACAGTA |
| mAPP ICD-R | TTAGTTCTGCATTTGCTCAA | |
| mTrkB ICD-F | 451-821 | CTCCTGCTCAAGTTGGCGAG |
| mTrkB ICD-R | CTAGCCTAGGATATCCAGGT | |
| mP75 ICD-F | 272-427 | TATATTGCTTTCAAGAGATG |
| mP75 ICD-R | TCACACAGGGGACGTGGCAG | |
| mLrp1 ICD-F | 4444-4545 | TGGTATAAGCGGCGAGTCCG |
| mLrp1 ICD-R | CTATGCCAAGGGATCTCCTA | |
| mDag ICD-F | 771-893 | ATCTGCTATCGCAAGAAGAG |
| mDag ICD-R | TTAAGGGGGAACATACGGAG | |
| mEphA4 ICD-F | 567-986 | TTTGTGATCAGCCGAAGACG |
| mEphA4 ICD-R | TCAGACAGGAACCATCCTGC | |
| mNG2 ICD-F | 2250-2327 | TACCTCCGCAAACGCAACAA |
| mNG2 ICD-R | TCACACCCAGTACTGGCCAT | |
| mCdh2 ICD-F | 744-906 | GTATGGATGAAACGGCGGGA |
| mCdh2 ICD-R | TCAGTCGTCACCACCGCCGT | |
| mHes1-F | 1-282 | ATGCCAGCTGATATAATGGA |
| mHes1-R | TCAGTTCCGCCACGGTCTCC | |
| mStat3-F | 1-722 | ATGGCTCAGTGGAACCAGCT |
| mStat3-R | TTATTTCCAAACTGCATCAA | |
| 3. Primers for luciferase assay | ||
| Promoter | Region | Sequence 5′-3′ |
| mStat3 promoter-F | −1839∼TSS∼261 | CGTAGTTAAGAGCACTGCTC |
| mStat3 promoter-R | TCCCGAGGGCCGCCTGGCCT | |
| mStat3 promoter fragment 2-F | −1338∼TSS∼261 | GCAGAGTTCATTCTCTATGA |
| mStat3 promoter fragment 2-R | TCCCGAGGGCCGCCTGGCCT | |
| mStat3 promoter fragment 3-F | −834∼TSS∼261 | AACTTGAACTTGAATTTTTA |
| mStat3 promoter fragment 3-R | TCCCGAGGGCCGCCTGGCCT | |
| mStat3 promoter fragment 4-F | −213∼TSS∼261 | TTTCAGCAGGACATTCCGCT |
| mStat3 promoter fragment 4-R | TCCCGAGGGCCGCCTGGCCT | |
| hGFAP promoter-F | −1702∼TSS∼−5 | CATATCCTGGTGTGGAGTAG |
| hGFAP promoter-R | GGGTGCCCCTGGCAACACCC | |
