Representative benchmark of temporal and spatial isolation of calcium activity. A, 3D laser-scanning confocal image (left) of a VTA slice from a live-cell calcium imaging dataset (right). B, Raw calcium activity from neurons 7, 8, and 9 (shown in A) were selected for their high degree of spatial proximity. C, Correlation matrix of calcium activity between neurons 7, 8, and 9. D, No significant relationship between neuronal soma distance and calcium activity is observed in the representative dataset.