Chronic augmentation of eCB signaling increases motivation. a, Experimental design. b–d, Left, Mean (±SEM) value on each session, compared using a two-way RM ANOVA. Right, The overall (bars) and individual (lines; blue, female; red, male) mean collapsed across sessions, compared using a paired t test (**p < 0.01). JZL increased break point (drug: F_(1,36) = 9.30, p < 0.01; session, F_(5,180) = 1.64, p = 0.151; drug × session: F_(5,180) = 1.29, p = 0.269; b), total rewards earned (drug: F_(1,36) = 11.61, p < 0.01; session: F_(5,180) = 1.64, p = 0.177; drug × session: F_(5,180) = 0.89, p = 0.487; c), and total lever presses (drug: F_(1,36) = 8.99, p < 0.01; session: F_(5,180) = 1.96, p = 0.103; drug × session: F_(5,180) = 1.23, p = 0.296; d).