Testing expression stability of housekeeping genes. Real-time PCR determination of the expression of housekeeping genes, Gapdh, Rplpo, and Actb, in the hypothalamus (A-C), liver (D-F), BAT (G-I), and epididymal white adipose tissue (J-L). In all experiments, n = 5. SCc, Scrambled fed on chow; OEc, overexpression fed on chow.