Analysis of ARC scRNAseq data. A, dbMAP representation of 20,921 cells from the ARC and median eminence (ME) of adult mice (DOI: 10.1038/nn.4495). Each dot represents a single-cell transcriptome. Its distance from other cells represents phenotypic (transcriptional) similarity. Cells are colored by the main cell types defined in the adult ARC-ME (DOI: 10.1038/nn.4495). B, Dot plot visualization of Nhlh2 and main cell type markers (Vim, tanycytes; Gfap, astrocytes; Cx3cr1, microglia; and Rbfox3 and Meg3, neurons). As shown, Nhlh2 is selectively expressed by neuronal populations. C, dbMAP representation of ARC neurons. Cells are colored by neuronal subclusters previously defined, and non-ARC subpopulations are marked (*). D, Visualization of Nhlh2 expression in the dbMAP neuronal embedding. Nhlh2 is expressed by POMC, KISS1, OXT, and Rgs16 neurons (left). Markers of these neuronal subpopulations, such as Pomc and Kiss1, are consistently coexpressed with Nhlh2 (right). E, Visualization of Nhlh2 expression in the ARC by ISH of a coronal slice in a 56-d-old mouse brain, retrieved from the Allen Mouse Brain Atlas (https://mouse.brain-map.org/experiment/show/74657929).