Energy expenditure and BAT parameters in mice under simultaneous introduction of an HFD and induction of overexpression of NHLH2 in the hypothalamus. Energy expenditure (A,B); oxygen consumption (C,D); carbon dioxide production (E,F); light- and dark-phase locomotor activity (G); whole-day locomotor activity (H); BAT temperature (I,J); BAT microscopic morphology (K); and BAT transcript expression of Adgre1 (gene coding for F4/80) (L) and Tnfa (M) in mice submitted to the protocol depicted in Figure 6E. *p < 0.05 versus SCh. ^#p < 0.05 versus OEh. EE, Energy expenditure; KDh, knockdown fed an HFD; OEh, overexpression fed an HFD; SCh, scrambled fed an HFD. A-H, K-M, n = 5; I, J, n = 7. Mice fed on chow were also evaluated as shown in Extended Data Figure 7-1.