Behavioral changes induced by the overexpression of NHLH2 in the hypothalamus. Mice submitted to the protocol depicted in Figure 6E were evaluated in the open field test (A-C), elevated cross maze (D,E), tail suspension test (F,G), time spent on the treadmill (H), and maximum RPM in the rotarod test (I). *p < 0.05 versus SCh. ^#p < 0.05 versus OEh. KDh, Knockdown fed an HFD; OEh, overexpression fed an HFD; SCh, scrambled fed an HFD. A-C, F-H, n = 10; D, E, I, n = 5 or 6. Mice fed on chow were also evaluated as shown in Extended Data Figure 9-1.