Quantitative analyses of htau transgene expression in rT2 and rT3 mice. A, Quantitative comparison of mRNA levels (normalized to levels of Hprt1) of MAPT in the forebrain between 2-month-old rT2 and age-matched rT3 mice. The scatter plot with bar graph represents data of individuals plus group mean ± SD. B, Quantitative comparison of protein levels (normalized to levels of βIII-tubulin) of fl-htau in the forebrain between 2-month-old rT2 and age-matched rT3 mice. Left, Representative WBs using antibodies recognizing htau (tau-13, top) and βIII-tubulin (bottom). Right, A scatter plot with bar representing individual data plus group median ± interquartile range. C, Quantitative comparison of forebrain protein levels (normalized to levels of βIII-tubulin) of dephosphorylated fl-tau (htau plus mtau, +/−) and endogenous fl-mtau (−/−) for 2-month-old rT2 and age-matched rT3 mice. Left, Representative WBs using antibodies recognizing tau (T46, top) and βIII-tubulin (bottom). Right, A scatter plot with bar representing individual data plus group mean ± SD. Numbers of animals (for detailed gender distribution, see Table 1) used are shown in parentheses. ****p < 0.0001. a.u., arbitrary units. kDa, kilodaltons.