Quantitative comparison of levels of Δtau314 in rT2 and rT3 mice. A, Representative Immunoprecipitation (IP)/WBs. Proteins from rT2 and rT3 mice (htau^+/− tTA^+/−) (5- to 9-month-old) captured by tau-13 antibody were revealed using biotin-conjugated 4F3 antibody for the detection of Δtau314 (top). A non-Tg littermate (htau^−/− tTA^−/−) of rT2 mice was used as negative control. Synthetic (Syn.) Δtau314 (6 µl) was used as positive control. fl-htau was detected by reprobing the same blot with biotin-conjugated tau-5 antibody (bottom). B, Quantitative comparison of levels of Δtau314 (normalized to levels of fl-htau) in the forebrain between rT2 and rT3 mice. The scatter plot with bar represents individual data plus group mean ± SD. Numbers of animals (for detailed gender distribution, see Table 1) used are shown in parentheses. ****p < 0.0001. kDa, kilodaltons.