Table 2.
Primary antibodies used in this study
| Antibody | Host/isotype | Epitope | Source | Usagec |
|---|---|---|---|---|
| Tau-13 | Mouse, IgG1, κ | tau15-25 | BioLegend, catalog #835201; RRID:AB_2565341 | IP (∼10 µg), WB (1:30,000, final conc.: n.d.) |
| T46 | Mouse, IgG1 | tau395-432 | Thermo Fisher Scientific, catalog #13-6400; RRID:AB_2533025 | WB (1:10,000, final conc.: 50 ng/ml) |
| AT8 | Mouse, IgG1 | phosphorylated tau at Ser202 & Thr205 | Thermo Fisher Scientific, catalog #MN1020; RRID:AB_223647 | IF (1:1000, final conc.: 200 ng/ml) |
| MC1 | Mouse, IgG1 | conformation-dependent, tau7-9 & tau313 -322 | P. Davies gift; RRID:AB_2314773 | IF (1:1000, final conc.: n.d.) |
| CP13 | Mouse, IgG2b, | phosphorylated tau at Ser202 | P. Davies gift; RRID:AB_2314223 | IF (1:2000, final conc.: n.d.) |
| PHF-1 | Mouse, IgG1 | phosphorylated tau at Ser396 & Ser404 | P. Davies gift; RRID:AB_2315150 | WB (1:5000), IF (1:1500); final conc.: n.d. |
| Anti-βIII-tubulin | Mouse, IgG2b | C-terminus | Millipore Sigma, catalog #T8578; RRID:AB_1841228 | WB (1:200,000, final conc.: 5 ng/ml) |
| Biotin-conjugated 4F3 | Mouse, IgG2b, κ | taux−314 | Ashe laboratory | WB (1:45,000, final conc.: 36 ng/ml) |
| Biotin-conjugated tau-5 | Mouse, IgG1 | tau210-241 | Thermo Fisher Scientific, catalog #MA5-12805; RRID:AB_10978000 | WB (1:30,000, final conc.: 17 ng/ml) |
| HT7 | Mouse, IgG1, κ | tau159-163 | Thermo Fisher Scientific, catalog #MN1000; RRID:AB_2314654 | SWAWB (1:100), WB (1:5000), IF (1:10,000, final conc.: 20 ng/ml) |
| Anti-AMPAR 1 (GluA1) | Rabbit, IgG | proprietary | Cell Signaling Technology, catalog #13185S; RRID:AB_2732897 | WB (1:5000, final conc.: n.d.) |
| Anti-PSD95 | Rabbit, IgG | proprietary | Cell Signaling Technology, catalog #2507S; RRID:AB_561221 | WB (1:5000, final conc.: n.d.) |
| Anti-NeuN | Rabbit, IgG | N-terminus | Millipore Sigma, catalog #ABN78; RRID:AB_10807945 | IF (1:1000, final conc.: n.d.) |
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Amino acid residues are counted using the 2N4R human tau splicing isoform numbering system. Ser, Serine; Thr, threonine; conc., concentration; n.d., not determined; IP, immunoprecipitation; IF, immunofluorescence.
