Characterization of the four-step protein extraction protocol using human brain tissue. A, Selected brain tissue (0.5 mm3 of inferior temporal gyrus of a control brain) was subjected to serial extractions allowing the segregation of proteins based on their cellular compartmentalization. Examples of obtained segregation for human proteins with known compartmentalization are shown. B, Extraction profile for selected disease-related proteins. TIMP3, Tissue inhibitor of metalloproteinase-3; ERK, extracellular signal-regulated kinase; EB3, end-binding protein-3; NeuN, neuronal nuclei; GluN2, glutamate NMDA receptor subunit 2; PSD-95, postsynaptic density protein-95; Fyn, Fyn kinase; SNAP-25, synaptosomal-associated protein 25; VAMP-2, vesicle-associated membrane protein 2; sAPP/APP, soluble/full-length amyloid precursor protein; ALS, amyotrophic lateral sclerosis; PD, Parkinson's disease; Insol, insoluble.
