Effects of cooling temperatures on intrinsic electrophysiological properties of lumbar spinal ventral nerves. A, Sample traces represent RMP and APs recorded at 35°C (red), 24°C (black), and 15°C (blue). Recordings were performed under the whole-cell current-clamp configuration, and step currents were injected into the NR through the recording electrode. B-H, Bar graph represents recordings at NRs performed at 35°C (red bars, n = 8), 24°C (gray bars, n = 8), and 15°C (blue bars, n = 8) for nodal RMP (B), AP width (C), AP amplitude (D), AP threshold (E), time to AP threshold (F), AP rheobase (G), and membrane input resistance (H). Data are mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; one-way ANOVA with Tukey post hoc test.