MIF treatment enhances DSFs in NA neurons from Naive but not SCI rats. A, Effects of different doses of MIF on DSF amplitudes in NA neurons isolated from Naive and SCI rats 1-4 months after surgery. Median DSF amplitudes (bars) are shown when measured at RMP and when artificially depolarized to −45 mV. Each circle represents the mean DSF amplitude measured in a 30 s recording from 1 neuron. Comparisons between each dose and vehicle (0 ng/ml) were performed by Kruskal–Wallis tests followed by Dunn's multiple comparison tests. Comparison among Naive and SCI groups during vehicle treatment was also performed using Kruskal–Wallis tests followed by Dunn's multiple comparison tests, with corresponding p values indicated on the figure. p < 0.05 was considered significant. B, Effects of different doses of MIF on the incidence of sampled neurons (same neurons as in A) with large DSFs (>5 mV) measured at RMP and when depolarized to −45 mV. Comparisons between each dose and vehicle were performed by Fisher's exact test. After Bonferroni correction for multiple comparisons, the significance level was *p < 0.012 in the Naive group and p < 0.017 in the SCI group. ***p < 0.00025 in the Naive group. Comparisons between Naive and SCI vehicle conditions were also performed with Fisher's exact test, with p < 0.025 considered significant after Bonferroni correction. For these comparisons, the significance level was ***p < 0.0005.