NA neurons dissociated from Naive rats exhibit MIF-dependent hyperactivity after exposure to conditioned medium bathing DRG cells dissociated from SCI rats. A, Experimental design. B–G, MIF-dependent effects of SCI conditioned media (SCI) versus either Naive conditioned media (N) or SCI conditioned medium plus Iso-1 (40 μm) after 1 h on nociceptors isolated from Naive rats. B, Increased incidence of neurons with OA at −45 mV but not at RMP, which was prevented by Iso-1 (Fisher's exact test, *p < 0.025, **p < 0.005 after Bonferroni correction). C, Lack of strong effect on mean RMP (Brown–Forsythe and Welch ANOVA test followed by Dunnett's T3 multiple comparison test, F_(2,56.49) = 2.5, p = 0.091). D, Lack of effect on mean AP voltage threshold (one-way ANOVA followed by Dunnett's multiple comparison test, F_(2,60) = 0.97, p = 0.38). E, Lack of effect on median rheobase (Kruskal–Wallis test followed by Dunn's multiple comparison test). F, Increased median DSF amplitude measured at −45 mV but not RMP, which was prevented by Iso-1 (Kruskal–Wallis test followed by Dunn's multiple comparison test). C–F, p < 0.05 was considered significant. G, Increased incidence of neurons with large DSFs, which was prevented by Iso-1 (Fisher's exact test, *p < 0.025, **p < 0.005 after Bonferroni correction).