Unique distribution and marker expression of ErbB4⁺ neurons in the OB. A, Schematic diagram of the mouse brain (top) and layer structure of the OB (bottom). B, Low-power confocal images represent the distribution of tdTomato-labeled ErbB4⁺ neurons across the OB. C, Zoomed view of the rectangle area of B. D, Summarized data showing the proportion of ErbB4⁺ neurons in different layers (n = 13 images from 3 mice). E, Confocal images represent the expression of tdTomato-labeled ErbB4⁺ neurons under different tamoxifen dosages. Images at 1, 3, and 6 doses were obtained at optimal thresholds (for details, see Materials and Methods). F, Summarized data showing the density of ErbB4⁺ neurons under different tamoxifen dosages (n = 5 images from 3 mice). G, Summarized data showing the fluorescence intensity of ErbB4⁺ neurons under different tamoxifen dosages (n = 5 images from 3 mice). H, Low-power confocal images represent the colocalization of ErbB4⁺ neurons (red) with PV⁺ neurons (green). I, Zoomed view of the rectangle area of H. J, K, Summarized data showing the proportion of PV⁺ neurons (J) and the PV/ErbB4 double-positive neurons account for total ErbB4⁺ neurons (K) in different layers (n = 13 images from 3 mice). L, Low-power confocal images represent the colocalization of ErbB4⁺ neurons (red) with TH⁺ neurons (green). M, Zoomed view of the rectangle area of L. N, O, Summarized data showing the proportion of TH⁺ neurons (N) and the TH/ErbB4 double-positive neurons account for total ErbB4⁺ neurons (O) in different layers (n = 7 images from 3 mice). P, Low-power confocal images represent the colocalization of ErbB4⁺ neurons (red) with CB⁺ neurons (green). Q, Zoomed view of the rectangle area of P. R, S, Summarized data showing the proportion of CB⁺ neurons (R) and the CB/ErbB4 double-positive neurons account for total ErbB4⁺ neurons (S) in different layers (n = 13 images from 3 mice). T, Low-power confocal images represent the colocalization of ErbB4⁺ neurons (red) with CR⁺ neurons (green). U, Zoomed view of the rectangle area of T. V, W, Summarized data showing the proportion of CR⁺ neurons (V) and the CR/ErbB4 double-positive neurons account for total ErbB4⁺ neurons (W) in different layers (n = 12 images from 3 mice). Scale bars: low-power image, 500 µm; zoomed view, 50 µm. ***p < 0.001.