High-magnification details of the distribution of glutamatergic subcortical (vGLUT2), GABAergic (GAD65/67) puncta and corticothalamic L5 axons and boutons. a–l, Comparison of vGLUT2 (a–d; green), tdTomato (L5-labeled cellls; e–h, magenta), and merge (i–l) dorsolateral Po, dorsomedial Po, ventral Po, and VPM domains in Rbp4-Cre;Ai14 mouse thalamus. m–x, Comparison of vGLUT2 (m–p; green), with GAD65/67 (q–t; blue), and its merge (u–x) in dorsolateral Po, dorsomedial Po, ventral Po, and VPM domains of the wild-type C57BL/6 mice. The sampled regions are indicated in Figure 4a–c. Scale bars: 10 µm (a–x).