Injection parameters applied to selectively inject BDA in brain regions that innervate Po
| Brain region | AP | ML | DV | Tip diameter (µm) | Current intensity (µA) | Time (min) | Cycle (s ON/OFF) |
|---|---|---|---|---|---|---|---|
| TRN | −1.5 | ±2.2 | −3.0 | 5–7 | 0.2–0.3 | 40–45 | 1 |
| ZI | −2.1 | ±1.5 | −4.0 | 6–8 | 0.3–0.5 | 40–45 | 1 |
| APT | −2.8 | ±1.1 | −2.6 | 8–12 | 0.8–1 | 40–45 | 1 |
| SC | −3.3 | ±1.0 | −2.0 | 8–10 | 0.8–1 | 20–30 | 1 |
| Gr | −0.2* | ±0.5 | −0.2* | 15–20 | 1–2 | 20–30 | 7 |
| Cu | −0.5* | ±1.0 | −0.2* | 15–20 | 1–2 | 20–30 | 7 |
| PB | −5.0 | ±1.1 | −2.5 | 10–15 | 1–2 | 20–30 | 7 |
| MVe | −5.8 | ±0.9 | −3.0 | 10–15 | 1–2 | 30–40 | 7 |
| PrV-VL | −5.4 | ±1.9 | −3.7 | 10–15 | 1–2 | 30–40 | 7 |
| PrV-DM | −5.4 | ±1.9 | −3.2 | 10–15 | 1–2 | 30–40 | 7 |
| SpVO | −5.8 | ±1.8 | −3.7 | 10–15 | 0.8–1 | 30–40 | 1 |
| SpVI | −7.2 | ±1.8 | −3.0 | 10–15 | 0.8–1 | 30–40 | 1 |
| SpVC | −7.7 | ±1.8 | −2.7 | 10–15 | 0.8–1 | 30–40 | 1 |
| Spinal cord | C1-C2 | ±0.5 | −0.3 | 12–14 | 1.5–2 | 20–30 | 7 |
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Stereotaxic coordinates for the anteroposterior (AP), mediolateral (ML), and dorsoventral (DV) levels with respect to bregma used for each structure are indicated. Asterisks indicate distance to the obex. Tip diameter (µm), current intensity (µA), time (min), and cycle (s ON/OFF) are indicated for each case. TRN: thalamic reticular nucleus, ZI: zona incerta. APT: anterior pretectal nucleus; SC: superior colliculus, middle and deep layers; Gr: gracilis nucleus; Cu: cuneatus nucleus; MVe: medial vestibular nucleus; PB: parabrachial nuclei; PrV-VL: principal trigeminal nucleus, ventrolateral portion; PrV-DM: principal trigeminal nucleus, dorsomedial portion; SpVO: spinal trigeminal nucleus, oral division; SpVI: spinal trigeminal nucleus, interpolar division; SpVC: spinal trigeminal nucleus, caudal division.
